Fig. 8

DS ameliorated neuropathic pain through mitophagy-mediated NLRP3 inflammasome inhibition. a Paw withdrawal threshold was measured by Von Frey test in both contralateral and ipsilateral paws, after administrating DS (10 mg/kg), DS (10 mg/kg) + 3-MA (2.5 mg/kg), and vehicle (0.9% normal saline) 9 days in CCI-induced model mice, respectively. b The analgesic effects of both DS (10 mg/kg) and DS (10 mg/kg) + 3-MA (2.5 mg/kg) treatment over four consecutive days post-injection. Data are expressed as means ± SEM (n = 7 mice in each group). Statistical significance was determined by two-way ANOVA followed by Tukey’s post hoc analysis where ^**P < 0.01 vs. CCI + Saline group, ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 vs. CCI + 10 mg/kg DS group. c, d Western blotting analysis of NLRP3 inflammasome components in spinal lumbar enlargement tissue. Data are expressed as means ± SEM (n = 3 in each group). Statistical significance was determined by one-way ANOVA followed by Tukey’s post hoc analysis where ^#P < 0.05, ^##P < 0.01 vs. Sham + Saline group; ^*P < 0.05 vs. CCI + Saline group; ^$P < 0.05 vs. CCI + 10 mg/kg DS group