Fig. 6

Glycolytic inhibitors reduce CM of activated microglial cell-induced MES23.5 neuroblastoma cell death. a BV-2 microglial cells were pretreated with 2-DG (600–1000 μM) for 30 min, followed by LPS (200 ng/mL) treatment for 6 h. The culture media was removed, and fresh medium was added. After 24 h of incubation, culture medium was added to the MES23.5 cells. MES23.5 cells were incubated with CM for 24 hours, and then, the cell viability was accessed by MTT assay. Cell viability was determined by MTT assay. b Primary microglial cells were pretreated with 2-DG (600–1000 μM) for 30 min, followed by LPS (200 ng/mL) treatment for 6 h. The culture media were removed, and fresh medium was added. After 24 h of incubation, culture medium was added to the MES23.5 cells. MES23.5 cells were incubated with CM for 24 h, and then, the cell viability was accessed by MTT assay. c MES23.5 cells were pretreated with 2-DG (125–1000 μM) before stimulation of MPP⁺ (1000 μM) for 24 h. Cell viability was determined by MTT assay. Data are presented as mean ± S.D. (n=3) and are representative of results obtained from three independent experiments. *p <0.05, **p <0.01, compared to the LPS or MPP⁺-alone group