Fig. 4

Validation of TBI-induced gene expression in astrocytes by dual in situ hybridization and immunofluorescence. A–D Representative confocal images demonstrating TBI-induced, astrocyte Serpina3n expression. Seven days post-FPI, Serpina3n expression co-localized with astrocyte marker, GFAP in A cortex, B corpus callosum, C hippocampus, and D thalamus (Scale bar, 50 um). E–L Quantification of number of GFAP+ astrocytes, and GFAP+ cells expressing Serpina3n in sham vs FPI mice in E, I cortex; F, J corpus callosum; G, K hippocampus; and H, L thalamus. Error bars depict mean ± SEM. Statistical analysis performed by two-sample, unpaired t tests. ***p < 0.001; **p < 0.01. n = 4 sham, 6 FPI mice