Fig. 7From: Celastrol exerts a neuroprotective effect by directly binding to HMGB1 protein in cerebral ischemia–reperfusionA, B Cel exhibited strong combining ability with HMGB1 protein in a concentration-dependent manner. C, D Cel almost completely competed for the binding of IAA-yne with HMGB1, and DTT reduced HMGB1. E, F The binding ability of cel to the HMGB1 protein was stronger than that of GA and Met. G Cel could bind to other sites of HMGB1 in addition to Cys 23, 45 and 106Back to article page