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Table 1 Primary antibodies

From: Celastrol exerts a neuroprotective effect by directly binding to HMGB1 protein in cerebral ischemia–reperfusion

Primary antibodies

Host

Dilution ratio

Supplier

MAP2

Mouse

1:500 (IF)

Abcam, United States

β-tubulin

Rabbit

1:500 (IF)

Abcam, USA

β-actin

Mouse

1:5000 (WB)

Affinity Biosciences, USA

PCNA

Rabbit

1:3000 (WB)

Proteintech Group, USA

NF-κB p65

Mouse

1:1000 (WB)

1:500 (IF)

Proteintech Group, USA

HSP70

Rabbit

1:1000 (WB)

1:500 (IF)

Abcam, USA

HMGB1

Rabbit

1:1000 (WB)

1:500 (IF)

Abcam, USA

RAGE

Rabbit

1:1000 (WB)

Proteintech Group, USA

TLR4

Rabbit

1:1000 (WB)

Proteintech Group, USA