Fig. 3

Effect of rA2 on TLR4/NFκB activation in leukocytes after TBI. A Representative gel images of Co-IP coupled with Western blotting analysis of the binding of TLR4 to TRIF, MyD88, and AXNA2 in isolated neutrophils of sham (S), TBI (T), and TBI + rA2 (T + rA2) mice at 12 h after TBI. B Representative gel images of Western blotting for p-P65, P65, and β-actin expression in isolated lymphocytes (Lym), monocytes (Mono), and neutrophils (Neu) of sham (S), TBI (T), and TBI + rA2 (T + rA2) mice at 12 h after TBI. C Quantitative analysis of p-P65 expression, n = 3. Data are expressed as mean ± SEM; **p < 0.01 compared to sham; ^#p < 0.05 compared to TBI