Fig. 1From: Microglia are involved in phagocytosis and extracellular digestion during Zika virus encephalitis in young adult immunodeficient miceBody weight change and viral RNA load in serum and brain of young adult mice infected with ZIKV. TRIF−/−xIPS-1−/− mice were infected intravenously with 1 × 105 PFUs of ZIKV strain PRVABC59 in 100 µL volume. Noninfected mice were used as controls. a Body weight changes of ZIKV-infected (○) and noninfected (■) mice. Results represent the mean ± SEM of 6 mice per group. b–c Subsets of mice were sacrificed on days 0 (noninfected), 3, 7, 10, and 14 post-infection and viral RNA load was determined in serum (b) and brain homogenates (c) by reverse transcriptase ddPCR. Results are the mean ± SEM of 5–6 mice per time point. The dotted lines represent the limit of detection of the reverse transcriptase ddPCR assay. ND, not detected. Statistical analyses were performed using a one-way analysis of variance with Tukey’s multiple comparison test. Results that are statistically different between indicated groups are shown as follows: * P < 0.05; *** P < 0.001Back to article page