Fig. 1

Schematic outlining the study design. The study consisted of 3 groups: control (vehicle, n = 9), LPS+vehicle (n = 8) and LPS+IL-1Ra (n = 9). The dashed vertical lines show the timing of LPS/vehicle infusions. The vertical green lines show the timing of IL-1Ra infusions (13 mg/kg) which were started 1 h after LPS infusions. Controls received an equivalent volume of vehicle (saline) during the infusion periods. Continuous physiological recordings were performed throughout the experimental period. Fetal preductal arterial blood was collected immediately before LPS or vehicle infusions and + 2 and + 6 h thereafter for measurement of cytokine levels and blood biochemistry. At 96 h, brains were collected for histological assessment. Numbers indicate regions of interest (ROIs) for assessment of the periventricular (1, 2), first (3, 4) and second (5, 6) intragyral white matter tracts, caudate nucleus (7, 8), putamen (9, 10), cingulate cortex (11, 12), first (13, 14) and second (15, 16) parasagittal cortices and the lateral cortex (17, 18)