Fig. 5
From: TLR7 is expressed by support cells, but not sensory neurons, in ganglia
Immunohistochemistry with TLR7 antibodies reveals TLR-independent labeling of small- and medium-sized sensory neurons. Mouse dorsal root ganglia and vagal ganglia were collected from wild-type mice and TLR7 KO mice, and paraffin sections were processed for immunohistochemistry. Both TLR7 antibodies (TLR7 Ab-1, Novus; TLR7 Ab-2, Abcam) labeled small-to-medium-sized neurons (brown) in A dorsal root ganglia and B vagal ganglia in both wild-type and TLR7 KO mice, indicating that this antibody is not specific for TLR7. C An identical staining pattern for TLR7 (brown) was detected in guinea pig dorsal root ganglia and nodose and jugular ganglia. Scale bar = 50 μm. Blue = hematoxylin nuclear stain. N = 4, representative images are shown