Fig. 5

NPSLE activates microglia and induces the engulfment of neurons. A The percentage of microglia (CD45^loCD11b⁺) in the hippocampus of control and MLR/lpr groups, n = 6. B The MFI of CD86 on microglia of control and MLR/lpr groups was detected by flow cytometry, n = 6. C The MFI of LAMP1 in microglia of control and MLR/lpr groups was detected by flow cytometry, n = 6. D Western blot quantification of LAMP1, CD86, and IBA-1 in the hippocampus of control and MLR/lpr groups, n = 4. E The expression levels of CD68 (green), IBA-1 (red), and their colocalization (yellow) in the hippocampus of control and MLR/lpr groups were detected by IF staining; Bar = 20 μm, n = 4. F Western blot quantification of PSD95 and VGLUT1 in the hippocampus of control and MLR/lpr groups, n = 4. J The expression levels of PSD95 (green), IBA-1 (red), and their colocalization (yellow) in the hippocampus of control and MLR/lpr groups were detected by IF staining; Bar = 20 μm, n = 4. Data are presented as mean scores ± SEM, *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001