Fig. 2

Peripheral LPS treatment does not alter neuronal densities in hippocampus or prefrontal cortex. a Mouse brain slice stained for nuclei (with DAPI, green) and for neuronal nuclei (with anti-NeuN antibodies, magenta). Regions of interest for quantification in CA1, CA3 and cortex are indicated (scale-bar 500 μm). NeuN⁺ neuronal densities in anatomically matched sections of b CA1 hippocampus, c CA3 hippocampus or d motor cortex of wild-type (WT) and P2ry6^−/− mice following i.p. injection of PBS (vehicle) or LPS. Mean width of e CA1 hippocampus and f CA3 hippocampus of same mice. Each data point represents one animal and error bars represent mean ± SEM. Statistical analysis was performed using two-way ANOVA, followed by Sidak-adjusted post-hoc tests, but there were no statistically significant differences