Fig. 2

Glycogen mobilization suppresses A1-like astrocyte formation and accelerates A2-like astrocyte formation during OGD/R. a Verification of GP overexpression in cultured astrocytes (ki-GP) using immunoblotting (n = 6). Vehicle represents unaffected astrocytes. Ve represents astrocytes affected by blank lentiviruses. b Heat map of relative mRNA levels of 12 target genes of A1-like astrocyte and A2-like astrocyte at 72 h following reoxygenation, determined by RNA sequencing (n = 4). c Astrocytic C3 levels at different timepoints before and after OGD/R challenge, as measured by immunoblotting (n = 6). The relative optical density was calculated by dividing the density of the C3 band by that of the corresponding β-tubulin band and then normalizing to the control group. d Astrocytic S100A10 levels at different timepoints before and after OGD/R challenge, analyzed by immunoblotting (n = 6). *P < 0.05, **P < 0.01, ***P < 0.001. One-way ANOVA followed by LSD post hoc analysis for a. Independent t test for b. Two-way ANOVA for c, d