Fig. 7

GPR39 CRISPR abolished anti-inflammatory effects of TC-G 1008 via GPR39/SIRT1/PGC-1α/Nrf2 signaling pathway at 48-h post-HIE. A Representative western blot bands. B–H Densitometric quantification of GPR39, SIRT1, PGC-1α, Nrf2, IL-1β, IL-6 and TNF-α in the ipsilateral hemisphere showed that TC-G 1008 treatment further upregulated the levels of GPR39, SIRT1, PGC-1α, and Nrf2, leading to less pro-inflammatory cytokines of IL-1β and TNF-α. GPR39 CRISPR significantly abolished such effects when administered with TC-G 1008. *p < 0.05 vs. sham; ^#p < 0.05 vs. HIE + vehicle; ^@p < 0.05 vs. HIE + TC-G1008 + control CRISPR, data are represented as mean ± SD, n = 6 for each group