Fig. 6

RNAseq of AAV-Gas6 treated APP/PS1 mice microglia reveals an enrichment of transcriptional signatures related to interferon response. A PCA plot of microglial samples. B Heatmap showing significantly DEGs between microglia isolated from AAV-Gas6 treated APP/PS1 mice and AAV-control hippocampi. Colors are shown in logarithmic scale. C Volcano plot with significantly downregulated genes (shown in red) and significantly upregulated genes (shown in green). Genes with a p-value < 0.001 are labeled. Vertical line is the p = 0.05 cutoff and horizontal lines show log2FoldChange = − 1 or 1. D Overrepresentation test of significantly upregulated genes through clusterProfiler package shows enrichment for interferon-gamma and beta response related pathways. E, F CXCL13 and CCL2 ELISA on hippocampal protein lysates from Gas6 and control-treated mice. Two-way ANOVA followed by Tukey post-hoc **p = 0.0018, ^#p = 0.0878