Fig. 3

Induction and antiviral activity of IFITs. A hBMECs were treated with poly I:C at different concentrations, and IFIT mRNA expression was measured. B–D hBMECs were infected with JEV P3 or heated-inactivated JEV P3 (heated-JEV P3, 94 °C, 15 min) or treated with poly I: C for different time points, and IFIT expression was measured by performing RT-qPCR (B) and Western blotting (C, D). E, F HEK-293T cells were transfected with IFIT1, IFIT2, IFIT3, and IFIT5 expression plasmids or empty vector plasmids at the indicated doses following infection with JEV P3. Then, the JEV-C gene was analyzed by RT-qPCR (E), and the JEV-E protein was measured by performing an immunofluorescence assay (F). Data are represented as the mean values ± SEMs from three independent experiments. **p < 0.01; ****p < 0.0001; ns: not significant