Fig. 7
From: Type I/type III IFN and related factors regulate JEV infection and BBB endothelial integrity
IFITs are nonessential in hBMEC barrier integrity. A, B The expression of IFITs was assessed by RT-qPCR (A) and Western blotting (B) in IFIT-knockdown hBMECs under JEV infection. C After serum-free starvation, cell viability was determined in IFIT-knockdown hBMECs. D, E IFIT-knockdown hBMECs were seeded onto the upper chamber of the Transwell plate until confluent. At the indicated times, the TEER (upper graph) was measured without treatment (D) or with rhIFN-β and rhIFN-λ1 protein treatment prior to the addition of JEV-CM (E). Data are represented as the mean values ± SEMs from three independent experiments. **p < 0.01; ****p < 0.0001