Fig. 3

Cumulative line graphs and corresponding area under the curve (AUC) bar graphs, representing the effects of aging and neuroinflammation on Iba-1⁺ microglial cell arborization complexity in the medial septum (MS), measured by Sholl analysis. Quantitative analysis of Iba-1⁺ microglia arborization complexity, including A intersections (n), B length (µm), C surface area (µm²), D volume (µm³), E average diameter (µm), and F nodes (n) of young (n = 30), adult (n = 30) and old (n = 30) mice from control and GFAP-IL6 cohorts. Data presented as mean ± SEM for AUC graphs and analysed with one-way ANOVA followed by Tukey’s post hoc tests. Post hoc effects of ‘genotype’ are represented in asterisks (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001); post hoc effects of ‘age’ are represented by hash symbols (^#p < 0.05, ^##p < 0.01, ^###p < 0.001, ^####p < 0.0001)