Fig. 9

The impact of aging and chronic neuroinflammation on the active membrane properties of cholinergic neurons in the MS. A Example action potential trace, detailing the parameters of spike amplitude and time to spike. B–D Box plots depicting the intrinsic active properties of the cholinergic neurons in the MS of control and GFAP-IL6 mice at young (light grey), adults (pink), and old (dark red) groups. The properties shown are rheobase (B), time to spike (C), spike amplitude (D). Data are shown as box plot, the box upper and lower limits are the 25th and 75th quartiles, respectively. The whiskers depict the lowest and highest data points, the horizontal line through the box is the median and the + sign represents the mean, two-way ANOVA, with Tukey’s post hoc test. Post hoc effect of ‘genotype’ indicated by asterisks, (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001); post hoc effect of ‘age’ indicated by hash symbols (#p < 0.05, ##p < 0.01, ###p < 0.001, ####p < 0.0001)