Fig. 2
Activation of the splenic nerve by splenic nerve stimulation (SpNS) elicits a NE voltammetry signal in the spleen. a NE voltammetry signal elicited by SpNS (10 s duration, 500 µs pulse width, 10 Hz pulsing frequency) or sham stimulation. Top and bottom panels similar to those of Fig. 1c. Dashed vertical line indicates the onset of SpNS. b Oxidation charge (Qo) vs. stimulation intensity in 3 animals, each represented by a different color; lines represent least square fits on data from each animal and r2 is the respective coefficients of determination. c Left panel: time course of NE peak oxidation current (io) in response to SpNS, in reserpine- and vehicle-treated mice. Right panel: Qo in response to SpNS in reserpine-treated vs. vehicle-treated animals (n = 5 in each group). p by unpaired t test. d Left panel: time course of io in response to SpNS recorded in the same animal before and after the topical application of lidocaine on the splenic nerve. Right panel: Qo before and after lidocaine application, in 5 animals. p by paired t test