Fig. 5

Post-SCI neuroinflammation in the hypothalamic PVN and hippocampal DG regions. A Representative images showing GFAP and IBA1 immunofluorescence staining in the hippocampal DG after SCI. Scale bar = 200 µm. A is the sham group, b is the T3-SCI group, and c is the T10-SCI group. B, C Quantitative analysis of the data in Figure A; all data are presented as the mean and standard deviation (SD); n = 4. B Number of GFAP + positive cells; (C) number of IBA1 + positive cells. C Representative high-magnification images of GFAP, IBA1, and PSD95 immunofluorescence staining in the hippocampal DG region after SCI. Scale bar = 50 µm. A is the sham group, b is the T3-SCI group, and c is the T10-SCI group. One-way and two-way ANOVA followed by Tukey’s multiple comparison test were used to analyse differences between groups. *p < 0.05, **p < 0.01, ***p < 0.001