Fig. 6

Differential gene expression in the T3 spinal cord and neuroinflammation after T3-SCI. A Volcano plot of DEGs between the sham group and the T3-SCI group; green indicates upregulated DEGs, and red indicates downregulated DEGs. B GO enrichment analysis of upregulated DEGs in the spinal cord after T3-SCI. C GO enrichment analysis of downregulated DEGs in the spinal cord after T3-SCI. D Representative images showing GFAP and IBA1 immunofluorescence staining in the T1 spinal cord after SCI (a is the sham group, b is the T3-SCI group, and c is the T10-SCI group). Scale bar = 100 µm. E Quantitative analysis of the IBA1 + expression intensity in Figure A; all data are presented as the mean and standard deviation (SD); n = 4. F Quantification of the GFAP staining intensity in Figure A. All data are presented as the mean and standard deviation (SD); n = 4. One-way and two-way ANOVA followed by Tukey’s multiple comparisons test were used to analyse differences between groups. *p < 0.05, **p < 0.01, ***p < 0.001