Table 1 Gene abbreviations: Ripk2, receptor interacting serine/threonine protein kinase 2; Tnfα, tumor necrosis factor alpha; Il1β, interleukin 1 beta; Il6, interleukin 6; Cxcl1, C–X–C motif chemokine ligand 1; Ccl2, chemokine C–C motif ligand 2; Mmp9, matrix metalloproteinase 9; Ly6g, Lymphocyte antigen 6 complex locus G6D; Nos2, nitric oxide synthase; Ywhaz, tyrosine 3-monooxygenase/tryptophan 5-monoogygenase activation protein zeta
Gene | Primer forward sequence | Primer reverse sequence |
|---|---|---|
Ripk2 | CGCTGCTCGACAGTGAAAGA | TGCCCAAAAATTCAGGCTCAT |
Tnfα | AGACCCTCACACTCAGATCA | TCTTTGAGATCCATGCCGTTG |
Il1β | GACCTGTTCTTTGAAGTTGACG | CTCTTGTTGATGTGCTGCTG |
Il6 | AGCCAGAGTCCTTCAGAGA | TCCTTAGCCACTCCTTCTGT |
Cxcl1 | CCAAACCGAAGTCATAGCCA | GTGCCATCAGAGCAGTCT |
Ccl2 | CATCCACGTGTTGGCTCA | AACTACAGCTTCTTTGGGACA |
Mmp9 | GACATAGACGGCATCCAGTATC | GTGGGAGGTATAGTGGGACA |
Ly6g | TGGACTCTCACAGAAGCAAAG | GCAGAGGTCTTCCTTCCAACA |
Nos2 | GTTCTCAGCCCAACAATACAAGA | GTGGACGGGTCGATGGTCAC |
18s | CTTAGAGGGACAAGTGGCG | ACGCTGAGCCAGTCAGTGTA |
Cyc1 | CCAAAACCATACCCTAACCCT | CTGCTCACTGGCTACTGTG |
Ywhaz | TGTTCTAGCCTGTTTCCCCG | ACGATGACGTCAAACGCTTC |