Fig. 6

Gene networks regulating brain endothelium dynamics are affected by the lack of cortistatin. Comparison of gene expression profiles in the dynamics from the basal (NX/NG) to ischemic and repairing (OGD-R) states in Cort^+/+ and Cort^−/− BECs. Each biological replicate (Rep) was pooled from 3 mice per genotype. a, d Volcano plots illustrating DEGs between BECs incubated under NX/NG for 24 h and BECs exposed to 4 h oxygen–glucose deprivation and 20 h reoxygenation (OGD-R) from Cort^+/+ (a) or Cort^−/− (d). Each dot represents one gene. Grey dots represent not significantly altered genes. The number of enriched (up, red) and decreased (down, blue) genes (FDR, p < 0.05) for OGD-R-derived BECs is shown in the legend. Full description of DEGs is in Additional file 2: Tables S7 and S8. b, e Heatmaps and unsupervised hierarchical clustering of 83 DEGs (b) and 20 DEGs (e) in BECs exposure to NX/NG vs OGD-R in Cort^+/+ and Cort^−/−, respectively. Expression values are represented in shades of red and blue, indicating expression above and below the median value across all samples. c, f GO terms for biological processes significantly overrepresented in BECs incubated under NX/NG vs OGD-R from Cort^+/+ (c) and Cort^−/− (f) mice. Data were grouped into similar gene sets and manually annotated into several networks, such as immune response, cellular matrix and remodelling, homeostasis, cell signalling and transcription, cell fate, brain endothelium dynamics, cell adhesion and migration, and cell metabolism. Red and blue bar-segments correspond to the numbers of upregulated and downregulated DEGs, respectively. All DEGs for each GO term are listed in Additional file 2: Tables S9 and S10. VEGF vascular endothelial growth factor, ECM extracellular matrix, MAPK mitogen-activated protein kinase, PPAR peroxisome proliferator-activated receptor