Fig. 6

IDE-KO microglia exhibit impaired cytokine profiling when activated with different stimuli. WT and IDE-KO primary microglial cultures were challenged with different stimuli for 18 h, and then cytokines released to the culture medium were measured. Stimuli tested include: A LPS (100 ng/ml), B IL-4 + IL-13 (20 and 50 ng/ml, respectively), C paraquat (50 µM) and D Aβ oligomers (1 µM). N = 3 samples per genotype and sex. Bars represent the mean ± SEM for each cytokine (unstimulated condition is not plotted in A and B). Statistical analyses were performed separately in males and females by two-way ANOVAs, considering the factors genotype and treatment. Individual differences were assessed by post hoc Holm–Sidak pairwise comparisons. *p < 0.05