Fig. 3

Regional and sex-specific transient increases in BrdU + cells following r-mTBI. A Representative images of the dorsal SGZ (white dotted line) for Sham and r-mTBI animals at PID1 and PID3 in males and females. Images taken of SGZ’s contra (middle column) and ipsi (right column) to the impact hemisphere showing BrdU + cells. Note the bilateral proliferative response to r-mTBI. B At PID1, there was an increase in BrdU + cells in the contra and ipsi-SGZ of male r-mTBI animals compared to Shams. Male r-mTBI animals showed a significantly higher number of BrdU + cells compared to female r-mTBI animals in contralateral and ipsilateral SGZ. In the ventral SGZ at PID1 (C) there was a significantly greater number of BrdU + cells in male r-mTBI animals in the ipsilateral hemisphere. There was a significant increase in BrdU + cells in the dorsal ipsilateral SGZ of female r-mTBI animals. D, E By PID3, there was no longer a significant difference in the number of BrdU + cells in either the dorsal or ventral SGZ of male r-mTBI animals. Images were taken at 10× (upper panels) and 40X (lower panels); scale bars are 50 µm and 25 µm, respectively. Data presented as mean ± SEM. Open circles in bar graphs represent individual animal data points