Fig. 5

Cell proliferation is not restricted to the SGZ at PID1. A Representative images of BrdU staining for the dorsal and (top in each pair) and ventral (bottom) hippocampus of Sham and r-mTBI animals at PID1 and PID3 in males and females. Contra (middle column) and ipsi (right column) are shown side by side to highlight the bilateral response that is especially robust in the ipsi hemisphere. B, C Quantification of this response by BrdU + cells in the hippocampus showed increased numbers of BrdU + cells in the dorsal hippocampus of r-mTBI males. This increase was significantly greater than Shams in the contra and ipsi hippocampus, though the increase in BrdU + cells in the ipsi hippocampus was significantly greater than the contra hippocampus of r-mTBI males. The ventral hippocampus did not show quite as large an increase and the response to r-mTBI was significantly higher in the ipsi hippocampus only. R-mTBI females showed a non-significant increase. D, E By PID3, there was a significant decrease in BrdU + cells in r-mTBI males compared to Shams. This was observed in the dorsal and ventral hippocampus. Representative images were taken at 10X, scale bar is 500 µm. Data presented as mean ± SEM. Open circles in bar graphs represent individual animal data points