Fig. 6

At PID1 a greater proportion of BrdU + cells were co-labeled with Iba1 + in r-mTBI males and females compared to Shams. A Representative images of triple labeling with BrdU (green, left column), Iba1 (red, second column), GFAP (blue, third column) and merged (fourth column) shown for the ipsilateral SGZ. Right column (expanded view of white rectangle from merged) shows representative BrdU + cells that were identified (white triangles in BrdU column) and examined for co-localization with Iba1 and/or GFAP. Scale bar is 10 µm. B, C A PID1, there was a greater proportion of BrdU + /Iba1 + /GFAP- cells in r-mTBI males and females compared to Shams. This indicates that this is likely an inflammatory response rather than exacerbated neurogenic proliferation. E, F At PID3, most BrdU + cells identified as BrdU + /Iba1-/GFAP- in r-mTBI animals, and there was no longer any difference in BrdU + cell phenotypes between r-mTBI and Sham animals. Scale bar is 10 µm. For triple labeling quantification, 1 dorsal and 1 ventral slice (approx. Bregma − 3.36 mm and Bregma − 5.28 mm, respectively) were chosen for imaging from n = 3 animals per group. Dorsal and ventral slices were averaged and presented as an n of 1; data are represented as mean ± SEM and individual points represent 1 animal. All BrdU + cells were identified in the DG from 20× stacks obtained using confocal microscopy. Scale bar: 100 µm