Table 1 Experimental antibodies
Primary antibody | Company | CAT# | RRID | Concentration used (%Serum) | Time in primary (h) | Secondary (source) |
|---|---|---|---|---|---|---|
Mouse anti-BrdU (DAB) | Millipore Sigma | MAB3424 | AB_94851 | 1:4000 (3%) | 16 | Goat anti Mouse (Millipore Sigma B6649) |
Rabbit anti-Ki67 (DAB) | Abcam | Ab15580 | AB_443209 | 1:1000 (5%) | 48 | Goat anti Rabbit (Millipore Sigma B8895 |
Sheep anti-BrdU (IF) | Abcam | Ab1893 | AB_302659 | 1:1000 | 16 | Goat anti Sheep Biotin (Invitrogen A24565) |
| Â | Â | Â | Â | Â | Â | Streptavidin Alexa Fluor 488 conjugate (ThermoFisher S11223) |
Rabbit anti-Iba1 (IF) | Wako | 019-19741 | AB_839504 | 1:1000 | 16 | Donkey anti Rabbit, Alexa Fluor 568 (ThermoFisher A10042) |
Mouse anti-GFAP (IF) | Millipore Sigma | G3893 | AB_477010 | 1:1000 | 16 | Donkey anti Mouse Alexa Fluor 647 (Millipore Sigma, AP192SA6) |