Human iPSC-derived glia models for the study of neuroinflammation

Stöberl, Nina; Maguire, Emily; Salis, Elisa; Shaw, Bethany; Hall-Roberts, Hazel

doi:10.1186/s12974-023-02919-2

Journal of Neuroinflammation

Table 1 Stimulation procedures and outcomes for microglia monocultures

From: Human iPSC-derived glia models for the study of neuroinflammation

Stimulus	Stimulus concentration	Stimulus time	Method	Measure of inflammation	References
LPS	10 ng/mL	4 h	RT-qPCR	Increased IL1B, IL6 Decreased CX3CR1, TREM2, CD33, CSF1R	[66]
	10 ng/mL	4 h	ELISA	Increased IL-6	[66]
	50 ng/mL	24 h	RT-qPCR	Increased IL1B, CCL2, TNFAIP3	[68]
	100 ng/mL	3 h	RT-qPCR	Increased IL1B, IL10, NLRP3	[67]
		3 h	Western Blot	Increased NLRP3	[67]
		4 h	RT-qPCR	Increased IL1B Decreased CX3CR1, TREM2, CD33, CSF1R	[66]
		4 h	ELISA	Increased IL-6, IL1B	[66]
		8 h	RT-qPCR	Increased IL1B	[69]
		18 h	RNA sequencing	Increased IL1B, TNF, IL6	[70]
		18 h	RT-qPCR	Increased IL1B, IL6	[70]
		24 h	Multiplexed ELISA	Increased TNF, IL-6, IL-8, IL-10, IL-1α, CCL2, CCL4, CXCL10, CCL17	[53]
			Multiplexed ELISA	Increased IL-1β, IL-6	[71]
			Profiler human cytokine kit	Increased TNF, Serpin E1, IL-8, IL-6, IL-1Rα, IL-1β, ICAM-1, CXCL10, CXCL1, CCL5, CCL2, CCL1	[58]
			Human magnetic Luminex assay	Increased IL-6, TNF, IL-10, CCL2, CCL3, CCL4, CCL5, CXCL1, CXCL2	[72]
			Cytometric bead array	Increased IL-6, MCP-1, IL-8, RANTES, GM-CSF, TNF	[60]
	1 µg/mL	24 h	RT-qPCR	Increased IL6	[73]
	1 µg/mL	24 h	ELISA	Increased IL-6	[73]
IFN-γ	10 ng/mL	24 h	Human magnetic Luminex assay	Measured 10 cytokines, no change detected	[72]
	20 ng/mL	24 h	Multiplexed ELISA	Increased TNF, IL-8, CCL2, CCL3, CCL4, CCL17	[53]
	20 ng/mL	24 h	Cytometric bead array	Increased IL-6, MCP-1, IL-8	[60]
LPS and IFN-γ	100 ng/mL LPS and 10 ng/mL IFN-γ	24 h	Human magnetic Luminex assay	Increased TNF, CCL2, CCL3, CCL4, RANTES	[72]
	100 ng/mL LPS and 20 ng/mL IFN-γ	24 h	RT-qPCR	Increased TNF and IL6	[62]
			RT-qPCR	Increased TNF, IL6, IL1B	[75]
			Cytokine antibody panel membrane	Increased MIPα/β, TNF, IL-6, IFN-γ, CXCL1, CXCL10	[62]
			Human cytokine array kit	Increased CCL2, MIPα, IL-6, IL-8, PA1-1, CXCL1, CXCL10	[75]
			Multiplexed ELISA	Increased IL-1β, TNF, IL-6	[71]
			Cytometric bead array	Increased IL-6, TNF, MCP-1, IL-8, RANTES, GM-CSF	[60]
		48 h	Cytometric bead array	Increased IL12p40, IL12p70, IL-6, IL-4, IL-10, IL-1RA, TARC, TNF, IFN-γ, IL-23	[76]
IL-1β	20 ng/mL	24 h	Multiplexed ELISA	Increased TNF, IL-8, CCL3, CCL4, CXCL10, CCL17	[53]
Amyloid-beta (Aβ_1–42)	1 µM	24 h	RT-qPCR	Increased TNF, IL6, IL1B, RELA	[73]
Amyloid-beta fibrils (Aβ-F)	0.2 µM	24 h	Cytokine array	Measured 36 human chemokines and cytokines, no change detected	[79]
Amyloid-beta oligomers (AβO)	3 µM	24 h	RT-qPCR	Increased TNF, IL6, IL1B	[78]
α-Synuclein monomers	750 nM	6 h	ELISA	Increased IL-6, TNF	[66]
α-Synuclein oligomers	750 nM	6 h	ELISA	Increased IL-1β, caspase-1, IL-6, TNF	[66]
α-Synuclein fibrils (αSYN-F)	0.5 µM	24 h	Cytokine array	Measured 36 human chemokines and cytokines, no change detected	[79]

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ISSN: 1742-2094

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