Human iPSC-derived glia models for the study of neuroinflammation

Stöberl, Nina; Maguire, Emily; Salis, Elisa; Shaw, Bethany; Hall-Roberts, Hazel

doi:10.1186/s12974-023-02919-2

Journal of Neuroinflammation

Table 2 Stimulation procedures and outcomes for astrocyte monocultures

From: Human iPSC-derived glia models for the study of neuroinflammation

Stimulus	Stimulus concentration	Stimulus time	Method	Measure of inflammation	References
TNF	10 ng/mL	24 h	RT-qPCR	Increased IL8, IL1B, IFNG, TNF, IL2, IL4, IL6, IL10	[88]
		24 h	Cytometric bead array	Increased IL-8, IL-1β, IFN-γ, TNF, IL-2, IL-4, IL-6, IL-10	[91]
		48 h	Cytometric bead array	Increased IL-8	[91]
		5 days	Bulk RNA sequencing	Increased IL6, C3, CXCL10, CXCL11 amongst others	[92]
			Immunostaining	Increased GFAP intensity	[88]
			Multiplex bead-based immunoassays	Increased GM-CSF, IL-1β	[94]
	30 ng/mL	7 days	Western Blot	Increased NF-κB phosphorylation	[90]
	50 ng/mL	1.5 h	Immunostaining	Increased NF-κB nuclear translocation	[88]
		5 h	Flow cytometry	Increased IL-8 and IL-6	[89]
		24 h	Cytometric bead array	Increased IL-8	[91]
		48 h	Cytometric bead array	Increased IL-8	[91]
		48 h	RT-qPCR	Increased CCL5, CXCL8	[93]
		7 days	Western blot	No change in GFAP expression	[93]
		7 days	ELISA	Increased IL-6	[93]
	100 ng/mL	7 days	Bulk RNA sequencing	Increased IL8, C3, CXCL10 and CXCL11 amongst others	[90]
	100 ng/mL	7 days	Western Blot	Increased NF-κB phosphorylation	[90]
IL-1β	10 ng/mL	5 h	Bulk RNA sequencing	Enrichment for inflammation-related GO terms	[89]
		5 h	Flow cytometry	Increased IL-6 and IL-8	[89]
		24 h	Cytometric bead array	Increased IL-8	[91]
		48 h	Cytometric bead array	Increased IL-8	[91]
		48 h	RT-qPCR	Increased CCL5, CXCL8	[93]
		5 days	Multiplex bead-based immunoassays	Increased IL-6, GM-CSF, TNF, IL-23, IFN-β, IFN-α	[94]
		7 days	Western Blot	Increased GFAP expression	[93]
		7 days	ELISA	Increased IL-6	[93]
	100 ng/mL	7 days	Bulk RNA sequencing	Increased IL8, C3, CXCL10 and CXCL11 amongst others	[90]
	100 ng/mL	7 days	Western Blot	No change in NF-κB phosphorylation	[90]
TNF and IL-1β	10 ng/mL TNF and 10 ng/mL IL-1β	1 h	RT-qPCR	Decreased GFAP	[96]
			Western blot	Decreased GFAP	[96]
			Immunostaining	Increased NF-κB nuclear translocation	[96]
		5 days	Multiplex bead-based immunoassays	Increased GM-CSF, TNF, IL-1α, IL-6, IL-10, IL-12, IL-23, IFN-β, IFN-α	[94]
		7 days	RT-qPCR	Increased CCL5, CXCL8, C3, LCN2	[96]
		7 days	ELISA	Increased IL-6	[96]
		24 h	Multiplex bead-based immunoassays	Increased C3a, MCP-3, I-TAC, GRO-a, sICAM-1, GM-CSF, IL-1RA, MIG, RANTES, IL-6, MCP-2, MIP-1a	[98]
IL-1α, TNF, and C1q	3 ng/mL IL-1α, 30 ng/mL TNF, and 400 ng/mL C1q	24 h	Multiplex bead-based immunoassays	Increased C3a, MCP-3, I-TAC, GRO-a, sICAM-1, GM-CSF, MIG, RANTES, IL-6, MCP-2, MIP-1a	[98]
			Western blot	No change in GFAP	[98]
			RT-qPCR	Increased TNF and IL-1β, decreased GFAP	[97]
			Whole proteome analysis	Enrichment of GO-terms related to immune responses, cytokine-associated signalling, and recruitment of peripheral immune cells following treatment	[147]
Amyloid-beta fibrils (Aβ-F)	0.2 µM	24 h	Cytokine array	Measured 36 human chemokines and cytokines, no change detected	[79]
α-Synuclein fibrils (αSYN-F)	0.5 µM	24 h	Cytokine array	Measured 36 human chemokines and cytokines, no change detected	[79]

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ISSN: 1742-2094

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