Fig. 5

CD59 patient sural nerve biopsy. A, B Immunohistochemical staining of CD59 (DAB, red) from a healthy control subject (A) and a CD59 patient (B). CD59-deficient patient staining of CD59 was completely absent in comparison to a healthy control subject where CD59 appears to localize in compact myelin. Scale bars = 50 μm, magnifications × 40, 1:400 dilution. C–H Hematoxylin and eosin (H&E) (C and D), epoxy-resin embedded semi-thin sections stained with toluidine-blue (E and F), immunohistochemical staining for MBP (G) and neurofilament (NF) (H). Staining displayed normal nerve fiber density without evidence of active axonal or myelin damage. Scale bars: C, D, G and H 50 μm; E and F 20 μm; magnifications: C, D, G and H ×40; E and F ×60. I Electron microscopy image, tissue from a CD59-deficient patient showing thin myelin sheaths relative to axonal diameter with a relatively high g-ratio, suggestive of remyelination. Scale bar 5 μm. small squares, myelin sheath lamellar structure. Scale bar 500 nm