Fig. 6

Localization of complement membrane regulatory proteins in murine teased fibers of sciatic nerve. Staining of WT teased fibers. CD55 (red), neurofascin (Nfasc, blue) and CD59 (green) (A), CD46 (red), neurofascin (Nfasc, blue) and CD59 (green) (B), Crry (red), neurofascin (Nfasc, blue) and CD59 (green) (C). Sections taken from a 4-month-old mouse. Immunolabeling procedure performed with the TSA method (A, C) and methanol and 0.1% triton (B). Scale bars 20 μm. CD55 staining was localized in Schmidt Lanterman incisures. CD46 staining was localized in the paranodes-juxtaparanodes, and Crry staining was localized in the paranodes and the internodes corresponding to compact myelin. White arrowheads indicate the nodes of Ranvier