Fig. 1
From: Mertk-expressing microglia influence oligodendrogenesis and myelin modelling in the CNS
Mertk is efficiently deleted from microglia in the presence of the Cx3Cr1 cre driver A Floxed Mertk mice were generated by the introduction of loxP sites on either side of exon 2 of the Mertk gene. An frt-flanked pGK-neomycin (neoR) cassette was also introduced for positive selection of embryonic stem cells. A diphtheria toxin A (DTA) cassette was introduced to allow for negative selection. B Representative flow cytometry plot (left) and corresponding histogram (right), indicating Mertk expression in Cx3Cr1+ve microglia generated from Mertk WT mice (black) compared with Cx3Cr1+ve microglia derived from Mertk cKO (red). C Widespread Mertk immunopositivity (magenta) is observed in the corpus callosum (dashed lines) of Mertk WT mice following 5 weeks of cuprizone-challenge to induce Mertk expression. Conversely, little to no Mertk expression is observed in the corpus callosum of Mertk cKO mice. Hoechst-labelled nuclei in blue. Scale bar represents 50 µm