Fig. 4

Altered mature oligodendrocyte production in the absence of microglial Mertk during development. A Representative immunofluorescence images of P28 Mertk WT (left) and cKO (right) corpus callosum tissue stained for the OPC marker PDGFRα and mature oligodendrocyte marker CC1 (yellow), EdU (magenta) in addition to nuclear Hoechst stain (cyan). Arrowhead indicates EdU⁺/CC1⁺ cell. Scale bar corresponds to 50 µm. B The density of PDGFRα^+ve OPCs was similar between genotypes. C A trend towards a reduction in the density of proliferating (Edu^+ve) PDGFRa^+ve OPCs was observed as well as a trend towards a reduction in the proportion of proliferating OPCs (D). E The density of CC1^+ve cells/mm² was not different between genotypes; however, the density of newly generated mature oligodendrocytes (CC1^+ve Edu^+ve) was increase twofold at P8 (F). G Similarly, the proportion of newly generated CC1⁺ve mature oligodendrocytes was increased at P8. Data represent mean ± SD. Statistical significance determined using 2-way ANOVA with Fisher’s LSD