Table 3 Secondary antibodies for immunohistochemistry
From: Mertk-expressing microglia influence oligodendrogenesis and myelin modelling in the CNS
Target species | Host species | Dilution | Conjugated fluorophore | Manufacturer, catalogue # |
|---|---|---|---|---|
Rabbit | Goat | 1:200 | AlexaFluor 488 | Jackson ImmunoResearch, West Grove, Pennsylvania, USA, 111-545-003 |
Rat | Goat | 1:200 | AlexaFluor 594 | Invitrogen, A11007 |
Mouse | Goat | 1:200 | AlexaFluor 594 | Jackson ImmunoResearch, 115-585-207 |
Rabbit | Goat | 1:200 | FITC | Jackson ImmunoResearch, 111-095,003 |
Goat | Donkey | 1:200 | TRITC | Jackson ImmunoResearch, 705-025-003 |
Rabbit | Donkey | 1:200 | AlexaFluor 488 | Invitrogen, A21206 |