Fig. 5

BAFF blockade with anti-BAFF antibody 10F4 protects hippocampal synapses and neurons in rr-EAE. A Overview of PSD95 (green), NeuN (red), and DAPI (blue) stained coronal section of brain from EAE mice showing the ROI of CA1, CA3, and dentate gyrus (DG) region used for quantification of PSD95 and NeuN integrated density to compare between the anti-BAFF antibody and control IgG-treated EAE mice. B–D Representative images of the CA1, CA3, and DG hippocampal region stained with PSD95 and NeuN from anti-BAFF treated, and IgG control-treated EAE mice brain coronal section. E–J Quantitative analysis shows significantly higher integrated density of PSD95 and NeuN in the CA1 region (E, F), CA3 region (G, H), and in the DG region (I, J) in anti-BAFF antibody-treated mice in comparison to the IgG control-treated mice. E–J Data were presented as box plots with the center line indicating the median, the box indicating the 25th and 75th percentiles, the whiskers indicating range, (min–max) and the dots representing all data points. The statistical analysis was conducted using Mann–Whitney U-tests. The significance level was set to *P < 0.05, **P < 0.01, ***P < 0.001. The scale bars for the images shown were 100 um. The number of EAE mice for the anti-BAFF antibody 10F4 group was 14 and for the IgG control group was 13