Fig. 6

BAFF blockade with anti-BAFF antibody 10F4 protects neurons and synapses near the meningeal infiltrates in rr-EAE. A Overview of PSD95 (green), NeuN (red), and DAPI (blue) stained coronal section of brain from EAE mice representing the ROI of brain parenchyma near leptomeningeal infiltrates used for quantification of PSD95 and NeuN integrated density and to count the NeuN + DAPI nuclei to compare between the anti-BAFF antibody and control IgG-treated EAE mice. B Representative images of the brain parenchyma near leptomeningeal infiltrates stained with PSD95 and NeuN from anti-BAFF treated, and IgG control-treated EAE mice brain coronal section. C Quantification shows significantly higher number of NeuN + DAPI nuclei in the cortex near leptomeningeal infiltrates in anti-BAFF antibody-treated group. D, E Quantitative analysis shows the significantly higher integrated density of PSD95 and NeuN in the cortex near leptomeningeal infiltrates in anti-BAFF antibody-treated group. C, D, E The box plots with the center line indicating the median, the box indicating the 25th and 75th percentiles, the whiskers indicating range, and the dots representing all data points. The statistical analysis was conducted using the Mann–Whitney U test, and P-values were derived from it. The significance level was set to *P < 0.05, **P < 0.01, ***P < 0.001. The scale bars for the images shown were 100 um. The number of EAE mice for the anti-BAFF antibody 10F4 group was 14 and for the IgG control group was 13