Fig. 1

ASK1-K716R inhibits the activation of ASK1 and JNKs/p38 signaling pathway 3 days following TBI. A Schematic diagram depicting homologous recombination repair to introduce the ASK1 Map3k5-e (K716R) site-specific mutation. B Quantification of ASK1 mRNA levels in the brain using qRT-PCR. n = 3 for WT Sham, n = 3 for ASK1-K716R Sham, n = 5 for WT TBI, n = 5 for ASK1-K716R TBI. C Quantification of ASK1 kinase activity in TBI mice using an ASK1 kinase activity detection kit (n = 6/group). D, E Representative Western blot bands (D) and quantification (E) of ASK1 and p-ASK1 protein levels in the ipsilateral hemisphere 3 days following TBI (n = 6/group). F–H Representative Western blot bands (F) and quantification (G, H) of p-ASK1/ASK1, p-JNKs/JNKs, p-p38/p38 protein levels in the ipsilateral hemisphere 3 days following TBI (n = 6/group). All data from male mice are presented as the mean ± SD. One-way ANOVA test and Bonferroni post hoc (B, E, G, H) and Student’s t test (C). * p < 0.05, *** p < 0.001, ns, no significance, as indicated