Fig. 5

ASK1-K716R improves brain tissue loss and white matter integrity 35 days following TBI. A Representative images of NeuN⁺ staining. Scale bar, 3 mm. B Quantification of tissue loss area and tissue loss volume. n = 8/group. C Representative images of MBP (green), SMI32 (red), and NF200 (purple) immunostaining in the CTX, EC, and STR 35 days following TBI. Scale bar, 100 μm. D–F Quantification of the fluorescence intensity of MBP, SMI32/MBP, and NF200 in CTX, EC, and STR 35 days following TBI. n = 5 for WT Sham, n = 5 for ASK1-K716R Sham, n = 8 for WT TBI, n = 8 for ASK1-K716R TBI. G Representative images of the NOR with Caspr (red) and Nav1.6 (green) immunostaining at the corpus callosum 35 days following TBI. Scale bar, 10 μm. H–J Quantification of the number of NOR (I), length of paranodes (J), and length of paranode gap (K). n = 6/group. L Schematic diagram of illustrating the placement of the stimulus electrode, record electrode, as well as representative CAPs. L, M Quantification of N1 (L) and N2 (M) amplitudes under different stimulation currents ranging from 0 to 2000 μA. n = 7 for WT Sham, n = 7 for ASK1-K716R Sham, n = 10 for WT TBI, and n = 10 for ASK1-K716R TBI. All data from male mice are presented as the mean ± SD. One-way ANOVA test and Bonferroni (D–F, H–J, L, M) post hoc and Student’s t test (B). * p < 0.05, ** p < 0.01, *** p < 0.001, ns, no significance, as indicated