Fig. 6

ASK1-K716R ameliorates long-term neurological dysfunction following TBI. A The timeline of behavioral tests is depicted in the diagram. B Score of the modified Garcia score test. n = 10 for WT Sham, n = 10 for ASK1-K716R Sham, n = 12 for WT TBI, n = 12 for ASK1-K716R TBI. C Time taken to contact and remove the tape in the adhesive removal test. n = 6 for WT Sham, n = 6 for ASK1-K716R Sham, n = 10 for WT TBI, n = 10 for ASK1-K716R TBI. D Latency to fall in the rotarod test. n = 8 for WT Sham, n = 8 for ASK1-K716R Sham, n = 14 for WT TBI, n = 14 for ASK1-K716R TBI. E Spatial learning and memory assessed in the Morris Water Maze, including the time spent in the target quadrant and swimming velocity (34 d after TBI). n = 8 for WT sham, n = 8 ASK1-K716R sham, n = 12 WT TBI, n = 12 ASK1-K716R TBI. F Representative track heat maps and time spent exploring objects in the new object recognition test. n = 8 for WT Sham, n =6 for ASK1-K716R Sham, n = 10 for WT TBI, and n = 10 for ASK1-K716R TBI. G, H Representative traces and time spent interacting with empty cages, stranger mouse 1, and stranger mouse 2 in the social affiliation session (G) and the social novelty preference session (H). n = 8 for WT Sham, n = 8 for ASK1-K716R Sham, n = 10 for WT TBI, n = 10 for ASK1-K716R-TBI. All data from male mice are presented as the mean ± SD. Two-way ANOVA repeated measurement (B–E) and paired Student’s t test (F–H, right). * p < 0.05, ** p < 0.01, *** p < 0.001, ns, no significance, as indicated