Fig. 1

PLX3397 eliminates microglia in the mouse brain. a Diagram of experimental illustration of mice placed on PLX3397 for 7 days followed by flow cytometric and immunohistochemistry for microglial density. b Representative image showing gating strategy and dot plot of CD45^interCD11b⁺. c Quantification of CD45⁺ macrophage from single cell population from the brain. d Quantification of CX3CR1-GFP + population following PLX3397 chow for seven days. e Quantification of the percentage of CD45^interCD11b⁺ (microglia) population from CD45⁺ cell. f Quantification of P2RY12 expression from CD45^interCD11b⁺ population. Representative images of the cortex (g) and the hippocampus (h) showing microglia after seven days of PLX3397 in chow. i, j Quantification of microglial density from the cortex (i) and the hippocampus (j) per field of view. Data were analyzed with unpaired Student’s T-test, n = 4–6, and data represented by mean ± SEM, ***p < 0.001, ****p < 0.0001