Fig. 4

Tet-29 administration returns the inflamed BBB to a phenotype resembling healthy. Brains from female C57Bl/6J mice immunised for EAE and treated daily with 30 mg/kg of Tet-29 from disease onset (disease score ≥ 1) were collected and processed for analysis by confocal microscopy. a Blood vessel permeability was quantified by the ratio of CD31 area stained over albumin area stained. c, e VCAM-1 and ICAM-1 expression was quantified by the % vessel coverage. b, d, f Representative images taken from the cerebellum, blood vessels were visualised with rabbit anti-mouse CD31 (red and grey), and co-stained with either goat anti-mouse albumin (green), rat anti-mouse VCAM-1/CD106 (magenta), or rat anti-mouse ICAM-1/CD54 (cyan). Each data point represents the average of 4–6 regions of interest analysed per section from at least 3 sections per animal with n = 6–9 mice per group. Scalebars = 50 µm. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 by one-way ANOVA with Tukey’s multiple comparisons