Fig. 5

Tet-29 has high affinity for multiple immune cell types. Treatment-naïve healthy and EAE female C57Bl/6J mice were administered a single i.p. dose of BDP-Tet-29 (3 mg/kg; sulfated), unsulfated BDP-Tet-29 (3 mg/kg) or the equivalent volume of PBS (100 µl). The compounds were allowed to circulate for 45 min before tissue was collected and analysed by flow cytometry. BDP expression on immune cells in the blood (a–d) and spleen (e–h) was quantified by MFI and displayed as a percentage of the vehicle control. Data are pooled from 3 independent experiments and displayed as mean ± SEM with n = 5–15 mice per group, *p < 0.05, **p < 0.01 by two-way ANOVA with Sidak’s multiple comparisons. i Representative histograms comparing BDP staining on neutrophils of mice treated with either sulfated BDP-Tet-29 or unsulfated Tet-29 with untreated controls