Fig. 6

Tet-29 inhibits inflammatory T cell migration in in vitro barrier models. In vitro models of the ChP and BBB. Splenocytes stimulated with ConA (1 μg/mL) for 24 h were co-treated ± Tet-29 at 6 μg/mL for 48 h and then added to the upper well of a Transwell culture seeded with a monolayer of endothelial (a, b) or epithelial cells (c, d). Transwell cultures were incubated for 24 h, and the extent of lymphocyte migration was assessed by flow cytometry of the upper and lower chambers. Data are pooled from 3 independent experiments and displayed as mean ± SEM with n = 5–8 biological replicates per group, *p < 0.05, ***p < 0.001 by two-way ANOVA with Sidak’s multiple comparisons