Fig. 7

Tet-29 does not inhibit homeostatic CNS infiltration. Healthy, female, C57Bl/6J mice were treated with 30 mg/kg/day of Tet-29, 10 mg/kg/day of PI-88, or 5 mg/kg of αVLA4 monoclonal antibody every four days for 10–23 days total. At the end of treatment, the distribution of leukocytes in spinal cord (a–c), brain (d–f), and spleen (g–i) was analysed by flow cytometry. Data were pooled from up to 8 independent experiments and displayed as mean ± SEM (n = 3–32 per group; full details in Additional file 6: Fig S6). *p < 0.05, **p < 0.01, ***p < 0.001 by Kruskal–Wallis with Dunn’s multiple comparison test (a–i) and by two-way ANOVA with Tukey’s multiple comparisons to assess the overall effect of treatments (right-hand tables)