Fig. 1
From: Impairing Gasdermin D-mediated pyroptosis is protective against retinal degeneration
GsdmdI105N/I105N mutant characterisation. A Schematic of murine GSDMD with I105N substitution site highlighted B Crystal structure of N-terminal and C-terminal domain of GSDMD created as superimposition of Protein Data Bank (PDB) 6N9N and PDB 6AO3 X-ray structures using SWISS-MODEL. C Retinal expression of Gsdmd at 1, 3 and 5 days (5d) PD compared to DR controls as measured by qRT-PCR (p < 0.05, N = 4–5, ordinary one-way ANOVA with Tukey’s multiple comparison test). D Expression of inflammatory genes in the retina, as measured by qRT-PCR, in WT and GsdmdI105N/I105N mice following PD. The expression of IL-1β, C3, Ccl2 and Gfap were significantly downregulated in GsdmdI105N/I105N mice compared to WT controls (p < 0.05, N = 5). E Total IL-1β release from WT and GsdmdI105N/I105N whole retina post-5-day PD as measured by ELISA (p < 0.05, N = 4–6). F, G Representative images of GSDMD IHC of WT and GsdmdI105N/I105N retina post-5day PD (Scale bar = 50 µm) showing H reduced GSDMD+ cells in subretinal space of GsdmdI105N/I105N mice in comparison with WT post-PD (p < 0.05, N = 5)