Fig. 2
From: Impairing Gasdermin D-mediated pyroptosis is protective against retinal degeneration
GsdmdI105N/I105N mice have improved retinal function, reduced cell death and immune cell recruitment compared to WT controls when subject to 5 days of photo-oxidative damage. A Experimental paradigm showcasing mice under DR and PD conditions. Retinal ERG function is significantly higher for WT mice in comparison with GsdmdI105N/I105N in DR condition as showcased by B a-wave (p < 0.05, N = 5) with no significant change in C b-wave responses (p > 0.05, N = 5). However, when subject to 5d PD, GsdmdI105N/I105N mice have significant protection against degeneration compared to WT as indicated in the retinal ERG trace D a-wave (p < 0.05, N = 7) and E b-wave (p < 0.05, N = 7). GsdmdI105N/I105N mice also show reduced photoreceptor cell death as indicated by F TUNEL + counts (p < 0.05, N = 7), G Improved retention of photoreceptor rows (p < 0.05, N = 7) and H reduction in recruitment of IBA-1 + cells in response to damage (p < 0.05, N = 7) I, J Representative image of TUNEL+ assay and K, L IBA-1+ IHC. Scale bar = 50 µm