Fig. 3

Gsdmd^−/− CRISPR knockout characterisation. A Schematic of murine GSDMD with CRISPR G9 target region highlighted, and corresponding amino acid sequence decoded. B Retinal gene expression panel of inflammatory genes, as measured by qRT-PCR, in WT and Gsdmd^−/− mice following 5d PD (p > 0.05, N = 4). C IL-1β released from WT and Gsdmd^−/− retina post-5-day PD as measured by ELISA (p > 0.05, N = 4). D, E Representative image of GSDMD immunohistochemistry in WT and Gsdmd^−/− retinas post-5day PD. Scale bar = 50 μm