Fig. 4

Gsdmd^−/− mice have no significant change in retinal function, cell death and immune cell recruitment compared to WT controls when subject to 5-day photo-oxidative damage. A Experimental paradigm showcasing mice under DR and PD conditions. Retinal ERG function is significantly higher for WT mice in comparison with Gsdmd^−/− in DR condition as showcased by B a-wave and C b-wave responses (p < 0.05, N = 4–5). However, when subject to PD, both WT and Gsdmd^−/− mice show comparable levels of degeneration as indicated in the retinal ERG traces for D a-wave and E b-wave (p > 0.05, N = 4–5). Gsdmd^−/− mice also show comparable levels of photoreceptor cell death as indicated by F TUNEL⁺ counts (p > 0.05, N = 4–5), G photoreceptor rows (p > 0.05, n = 4–5) and H recruitment of IBA-1⁺ cells in DR condition and in response to damage (p > 0.05, n = 4–5) I, J Representative image of TUNEL assay and K, L IBA-1⁺ immunohistochemistry. Scale bar = 50 µm