Fig. 8
From: Impairing Gasdermin D-mediated pyroptosis is protective against retinal degeneration
GSDMD inhibition reduces release of pro-inflammatory cytokine IL-1β. A Experimental paradigm showcasing in-vitro siRNA inhibition followed by inflammasome activation of iBMDM. B Representative, western blots showing bands of 42 kDa for CASP-1, ~ 50 kDa for full-length GSDMD and ~ 30 kDa for cleaved GSDMD and 37 kDa for GAPDH reference protein, in iBMDM lysates. C Total IL-1β release from iBMDM post-LPS/ATP stimulation, as measured by ELISA (p < 0.05, N = 4). D EV concentration measured using NTA in the supernatant of iBMDM with GSDMD-specific siRNA compared to non-specific siRNA control, showing a reduction in EV number but no significant change (p > 0.05, N = 4)